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Sunday, May 10, 2009

NEOSIS, as committed


Just over the bow of my kayak it appears that the salt water at the head of the Oyster Pond on the Atlantic where Lynnda and I live, ends... like the development of neosis. In reality, there is a fresh water stream hidden there, that leads to a string of lakes with great fishing, eagles and all sorts of other flora and fauna... I want to use this blog posting to introduce you to the reality of neosis, the birth of cancer cells, and the potential of getting to fresh research if we get these videos into the right hands. Dr. Rajaraman and others have tried the conventional processes... no luck. Please read on...


As I have committed, I am forwarding several videos of the Neosis Process... the birth of cancer cells. The descriptions of each are a little technical, but really don't lend themselves to much more interpretation that the videos themselves don't give.


These are from Dr. Rajaraman's research in the 1998 time frame, and formed the basis of the papers that are referred to in the blog prior to this. You may need QuickTime Player to view these, it is available on the web, free. If you have further problems, just paste the first link below into your browser and it will take you to the Dal Server.


Please click on each of the highlighted areas below. The descriptions are for each video in order. You can double click on each video to have it cycle again. The sixth is time stamped, imaging watching this through a microscope for thousands of hours, then noticing this for the first time... feel free to pass these on... I am trying to gain attention to neosis and Dr. Rajaraman's work. I believe that there is important research here that is being buried.


VC1 - http://rajaraman.medicine.dal.ca/vc1.mov (582kb) Normal and tumor cells undergo pre-mature seneescence after exposure to genotoxins. A subpopulation of these Senescent Polyploid Giant Cells (SPGCs) undergo neosis, a novel mode of cell division, that is responsible for the birth and continuous growth of all cancers. This video clip demonstrates that the process of birth of cancer cell during neoplastic transfomation of mouse cell exposed to etoposide occurs via neosis. This is the process by which the transformed foci is formed in mouse cells after exposure to etoposide. Transformed focus formation assay is equivalent to tumorigenicity in vivo. The senescent polyploid giant cell (SPGC) or the neosis mother cell (NMC), being unable to divide by mitosis. has become polyploid and has produced several nuclear buds, which after being separated from the polyploid nucleus of the neosis mother cell have moved to the extremities of the giant mother cell, which will die after producng the daughter Raju cells. The latter divides by symmetric mitosis and mature into tumor cells..This NMC produced about 10 Raju cells, most of them 'walked' away from the field. Ony three of them are seen undergoing mitotic proliferatioon.
VC2 - Neotic Catastrophe in C3H10T1/2 cells (2,229 kb) About 10% of the SPGCs undergo neosis; some of them cannot complete neosis and die in the process. This is called neotic catastrophe, as opposed to the death of a cell that cannot complete mitosis, called mitotic catastrophe. In this example, the Raju cells are formed but they cannot externalize themselves and eventually die in side the mother cell.
VC3 - S/T Neosis in HTB11 cells (1,578 kb) This demonstrates that human metastatic neuroblastoma HTB11 cells spontaneously undrgo neosis and produce the nexxt generation of TumorRepopulating Raju cells (TRRCs) This demonstrates two points: (1) Tumor cells are not immortal; But they can escape senescnce via neosis; (2) The life cycle of a tumor cell consists of a near diploid symmetric mitotic phase alternating with the polyploid senescent phase, from which they escape death by undergoing neosis. This cycle is repeated several times during the growth of tumors.
VC4 - Mitosis of Raju cells (1,578 kb) This displays the symmetric mitotic proliferation of Raju cells in the processs of repopulating the tumor. It is known that during this proliferative phase cells accumulate additional mutations and/or epimutations, leading to the next senescent phase and neosis. This contributes to the heterogeneity of tumor cells, which are constantly subject to natural selection.
VC5 - Maturation & Clonogenicity Raju cells (1,578 kb) This video clip demonstrates the maturation of Raju cell derivatives during mitotic proliferation. Note the increase in cell size as the cslls are dividing by mitosis. This is considered a property of stem cells, which are incoporating G1 phase in their short cell cycle.
VC6 - Neosis during Spontaneus Transformation of P53-/-MEF/MGB cells. (9,873 kb) Mouse fibroblasts without p53 the major tumor supressor protein, are considered an ideal model to study spontaneous transformation, Accordingly, if neosis is involved in neoplastic transformation, we can expect neosis involved in the spontaneous transformation of mouse cells that lack p53 tumor suppressor gene. These p53-/- mouse fiibroblasts spontaneously enter senescent phase at the end of their diploid mitotic life span and virtually all of these SPGCs undrwent neosi to yiueld 50-70 ormore Raju cells that eventually filled the whole Petri plate. The video clip dmonstrates that these cells spontaneously undergoing neosis, over the course of several hours. Please note near the centre, a Raju cell (daeker than the background surrounded by a white halo) is attempting to detach itself by performing what we call a "birth dance". On the left lower corner, two cells that have externalizzed themselves are "walking" on the mother cell, attempting to move away. .

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Grad. Saint Mary's University, 1975, got into the medical device business initially in sales, then various management positions up to president, all in Medical Devices. I prefer therapy products over diagnostic, but they are all fun, and in a way have defined my life. I have now evolved, with help from my 35 year partner Lynnda with whom I now share every hour. I am into staying healthy, photography, kayaking, bicycling, gardening and two books a week. I wish I had gotten to this stage earlier!